Vol.7, No.2, March - April 2019
Current Research in Microbiology and Biotechnology is an international open access bi-monthly journal that covers all aspects of latest research in microbiology and biotechnology. Manuscripts are published soon after peer-review.
| 1. |
Research Article |
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| Pages |
1674-1680 |
Authors |
Haya Khalid, Maya Kumari and M. Nasim |
| Citation |
Haya Khalid et al. Current Research in Microbiology and Biotechnology 7(2) 2019: 1674-1680 |
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1674-1680
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ABSTRACT: The cellulase producing bacteria were isolated from Defence Institute of Bioenergy Research (Haldwani) farm yard manure (FYM) collected. A total of 72 isolates were isolated and screened, of which ten isolates showed measurable hydrolyzing zone on Congo agar media plates. Isolates were tentatively characterized on the basis of morphological and biochemical characteristics. LCDB 10 identified as Pseudomonas aeruginosa exhibited the maximum zone of clearance of 4 mm, implying maximum enzyme activity however LCDB 1, LCDB 2, LCDB 4 and LCDB 9 exhibited the minimum zone of clearance i.e. 2 mm respectively. The enzyme assays for two enzymes, filter paper cellulase (FPC) and cellulase were examined. The extracellular cellulase activities ranged from 0.39 to 0.72 U/mL for FPC and 0.2 to 0.40 U/mL for CMC cellulase assay. However the intracellular FPCase activities range from 0.41 to 0.69 U/mL and 0.23 to 0.72 U/mL for CMC cellulase assay. Optimization of different parameters i.e. temperature and pH was carried out for the production of cellulose. The optimum pH and temperature for cellulose activity produced by strain LCDB 10 were pH 9 and 50° C respectively. The enzyme was found to be stable at a wide range of pH ranging from 5-9 for LCDB 10. However for other strains it was found that the cellulase activity was declined with increasing pH.
KeyWords: Farm yard manure, cellulose degrading bacteria, cellulase, endoglucanase.
How to cite: Haya Khalid et al. Isolation, screening, characterization and optimization of cellulase production of cellulytic bacteria from farm yard manure. Curr Res Microbiol Biotechnol. 7(2) 2019: 1674-1680
| 2. |
Research Article |
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| Pages |
1681-1685 |
Authors |
May K. Ismael*, Mahmood R. Al-Haleem and Rawaa S. Salman |
| Citation |
May K. Ismael et al. Current Research in Microbiology and Biotechnology 7(2) 2019: 1681-1685 |
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1681-1685
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ABSTRACT: The present study evaluated the anti- Helicobacter pylori IgG, IgA and the role of virulence factor of H. pylori Vacuolating associated cytotoxin gene (Vac A) as a risk factors for CAD. The levels of serum IgG and IgA was done by indirect immunofluorescent (IIF) whereas Vac A measured by enzyme linked immunosorbent assay (ELISA). Ibn Al-Bitar specialist center for cardiac surgery laboratory and Ministry of Health/ Baghdad/ Iraq, between May and October 2018. Seventy Iraqi patients with CAD were enrolled in this study, their ages ranged between 40-84 years ; and 20 individuals as a control group which was divided into 2 subgroups: 10 apparently healthy volunteers (negative control) and the other subgroup contained 10 with normal coronary artery but had other heart disease except CAD (positive control). There were high significant differences (P< 0.01) of anti- H. pylori IgG between CAD patients and both positive and negative controls. 78.57% (55/70), 100% (10/10) and 0% (0/10) respectively. Also, a highly significant difference was observed in the serum level of anti H. pylori IgA between CAD patients and both positive and negative controls37.14 % (26/70), 30% (3/10) and 0% (0/10) respectively. There is significant difference in the mean value of Vac A antigen of CAD patients when compared to control group (1.299± 0.04), and (1.41±0.13) respectively. As well as a significant difference found between another CAD patient group as compared to control group (1.79±0.17), (1.41±0.13) respectively (P˂ 0.05). The results suggest a relationship between Helicobacter pylori infection and coronary artery disease.
KeyWords: Helicobacter pylori, Antibodies, Atherosclerosis, Coronary artery disease, Vac A.
How to cite: May K. Ismael et al. Seropositivity of Helicobacter pylori among Iraqi patients with Atherosclerosis and coronary artery disease. Curr Res Microbiol Biotechnol. 7(2) 2019: 1681-1685
