Current Research in Microbiology and Biotechnology

ABSTRACT: Out of 28 isolates of Pseudomonas aeruginosa from otitis media. The result revealed that the algD virulent gene was present in 21 isolates with 75 percentage. The gel electrophoresis showed that the molecular weight of algD gene was 1310 bp. DNA sequences of algD gene was done, and the results showed presence of some gene mutations like substitution, addition and deletion with 97% identity with the Refseq gene. From the other side, the results of identities of translated nucleotide sequence with the original sequence of amino acids revealed that there were effects of gene mutations on translation of the product protein.

KeyWords: Pseudomonas aeruginosa, algD gene, Sequencing.

How to cite: Rana M. Abdullah Al-Shwaikh et al. Nucleotide sequences of the Pseudomonas aeruginosa algD gene isolated from Iraqi patients with otitis media. Curr Res Microbiol Biotechnol. 5(3) 2017: 1062-1070

ABSTRACT: Bacteriocins are antibacterial proteins produced by bacteria and it's active against other strains bacteria which are closely related to the producing strains and some species from the same family. The aim of this research was to investigate the antibacterial activity of bacteriocin from clinical Proteus mirabilis isolates from Baghdad /Iraq. The antibacterial activity of bacteriocins from P. mirabilis isolates against different pathogenic species of Gram-negative bacteria (by well assay method) was investigated, however antibiofilm activity determined also by tissue culture plate method against pathogenic species befor and after biofilm formation. The results showed that bacteriocin was effective against pathogenic species in different concentrations by producing different inhibition zones. Results also revealed that bacteriocin had a broad antibiofilm activity on many pathogenic species of Gram-negative bacteria.In addition to these results it was observed that the effect of bacteriocin against preformed biofilm of Klebsiella was higher than the effect of it against other bacterial species while it was affected on mature biofilm of other bacterial species in addition to Klebsiella.

KeyWords: Bacteriocins, antibiofilm activity, Proteus mirabilis, Escherichia coli, Klebsiella pneumoniae.

How to cite: Alyaa Rzooqy Hussein et al. The antibiofilm activity of bacteriocin produced by Proteus mirabilis against some bacterial species. Curr Res Microbiol Biotechnol. 5(3) 2017: 1071-1077

ABSTRACT: To determine the relationship between Helicobacter pylori infection and skin disorders, sixty six patients who suffering from skin diseases include chronic urticarial (CU) and atopic dermatitis (AD) who attended at Dermatological Clinic/ Al-Numan Teaching Hospital from the beginning of October 2015 to the end of January 2016 with age (6-62) have been investigated and compared to twenty two samples of apparently healthy individuals were studied as control group. All the studied groups were subjected to measurement of anti-Helicobacter pylori IgG antibodies by enzyme linked immuno sorbent assay (ELISA) and detection of 16S rRNA and CagA genes by using singleplex and multiplex PCR methods. The results of current study revealed that there was a highly significant elevation (P<0.01) in concentration of H. pylori IgG antibodies in sera of the CU and AD patients compared with control group, also the results revealed that there was a significant elevation (P<0.05) in concentration of H. pylori IgG antibodies in sera of the CU patients compared with control group, and significant elevation (P<0.05) in concentration of H. pylori IgG antibodies in sera of AD patients compared with control group. The results of present study indicated that 26(66.67%) patients out of 39 CU patients were positive for both of 16S rRNA and CagA genes, while 13(33.33%) patients out of 39 CU patients were negative for those genes. Also, the results revealed that 19(70.37%) patients out of 27 AD patients were positive for both of 16S rRNA and CagA genes, whereas only 8(29.62%) were negative for both those genes comparing with control group which showed 1(4.54%) individuals out of 22 apparently healthy individuals were positive for both 16S rRNA and CagA genes, the statistical analysis was highly significant (P<0.01).

KeyWords: Chronic urticarial, atopic dermatitis, Anti-Helicobacter pylori Abs, 16S rRNA gene, CagA gene.

How to cite: Tasneem Z. Abd-Alrahman et al. Immunological and molecular detection of Helicobacter pylori in patients clinically diagnosed with chronic urticarial and atopic dermatitis. Curr Res Microbiol Biotechnol. 5(3) 2017: 1082-1087

ABSTRACT: Paint is a liquid or semi solid synthetic chemical solution used for the protective and decorative finishing of surfaces. Its components make it susceptible to microbial attack. The economic losses as a result of biodeterioration of outdoor wall surfaces especially those of buildings by fungi can be expensive as these deteriorated exterior surfaces have to be refurbished regularly. This study aimed at determining the fungi involved in the deterioration of painted outdoor walls. Outdoor paint samples for three different types of paint – emulsion, textured paint and oil paint – were collected from various buildings that showed signs of microbial attack. The fungi were isolated using Potato Dextrose Agar. Counts were determined using the dilution plating technique. Fungal identification was done using macroscopic and microscopic methods. The implicated fungi belonged to nine genera – Aspergillus, Penicillium, Fusarium, Alternaria, Curvularia, Mucor, Saccharomyces, Rhizopus and Cladosporium. Emulsion paints were found to be more susceptible to microbial attack having the a greater diversity of isolates and the highest total fungal counts while oil paints were considered least prone to microbial deterioration as the samples had the lowest total fungal counts and diversity. Emulsion paint showed mean total fungal counts of 5.1 x 104 – 6.2 x 104 while textured paint and oil paint had mean values ranging between 2.0 x 104 and 4.1 x 104 and 1.5 x 104 and 3.65 x 104 respectively. The highest frequency of occurrence of fungi across all the paint types was in the order, Aspergillus > Mucor > Penicillium > Rhizopus. The other isolates had frequencies below 50%.

KeyWords: Biodeterioration; Emulsion; Fungi; Oil Paint; Painted wall; textured paint.

How to cite: A. U. Osadebe et al. Study of Fungi associated with the biodeterioration of Painted Outdoor Wall Surfaces. Curr Res Microbiol Biotechnol. 5(3) 2017: 1088-1094

ABSTRACT: Twelve male rats, Albino Sprague Dawley rats, approximately age 42 day were used for this study that divided into 3 groups, Group A: treated orally with 20mg aspartame that dissolved in 1.5ml of sterile distilled water, Group B: treated orally with 20mg saccharin that dissolved in 1.5ml of sterile distilled water, Group C: control received equivalent quantity sterile distilled water for the same period. The results for test of blood as Triglyceride, Cholesterol, HDL, LDL and VLDL parameters measured showed 75±3.83, 70±2.90, 50±1, 12±0.9 and 20±1 (mg/dL) respectively at 0mg concentration of sweeteners, while it was 55.7±1.91, 71±2.81, 60±1.5, 11.5±0.5 and 21±0.5 (mg/dL) respectively at 20mg concentration of aspartame and it was 80±2.70, 73±2.95, 55±2, 12.2±0.6 and 20±0.7 (mg/dL) respectively at 20mg concentration of saccharine. All of Glucose, Total protein, Albumin, Globulin, Ind bilirubin, D bilirubin, T bilirubin, Blood urea, Uric acid and Creatinine parameter were measured in this test showed 132.0±5, 6.3±0.8, 2.5±0.1, 4±0.07, 0.10±0.1, 0.04±0.01, 0.14±0.02, 51.9±2.18, 3.6±0.05 and 0.50±0.03 (mg/dL) respectively at 0mg concentration of sweeteners, while it was 131.5±6, 6.6±0.5, 2.6±0.1, 4.4±0.1, 0.9±0.01, 0.04±0.02, 0.09±0.01, 47.2±1.67, 2.6±0.06 and 0.25±0.03 (mg/dL) respectively at 20mg concentration of aspartame and it was 133.1±5.2, 6.7±0.3, 2.55±0.1, 4.2±0.2, 0.11±0.01, 0.04±0.02, 0.09±0.02, 40.1±1.47, 2.9±0.05 and 0.21±0.02 (mg/dL) respectively at 20mg concentration of saccharine. Histological test results showed different change in liver, kidney and spleen tissues after treated with aspartame and saccharine.

KeyWords: Sweeteners, Aspartame, Saccharine, Blood, Living tissues.

How to cite: Mohammed A. Alsoufi et al. Effect of some artificial sweeteners consumption in biochemical parameters of rats. Curr Res Microbiol Biotechnol. 5(3) 2017: 1095-1099

ABSTRACT: Cytomegalovirus (CMV) is DNA virus, human have only known host like other members of herpes virus family cause latent infection and infect people on all age and socioeconomic group, is the most common cause of congenital infection because it occurs after both primary and recurrent infection in pregnancy. It is a virus from herpes and chickenpox virus family is a common case of infection and illness worldwide the Infection can be congenital (present at birth) or passed from an infected pregnant woman to her baby. The aim of the study is to evaluate the Cytomegalovirus IgG and IgM seropositivity among pregnant women, in addition to studying relations to the abortion factors. A total of 100 blood samples of pregnant women were collected from the private laboratory for Pathogenesis Analyses in Baghdad which obtained during the period from 1st September 2016 till the 31st of December 2016. Data was collected by using questionnaire sheet concerning their ages of pregnant women between (16-45 years), place of residence (urban, rural), living status (good, medium, bad), a pregnancy of women (pregnant, non-pregnant), abortion (primary, recurrent). Each blood was analyzed for the presence of CMV IgG antibodies and IgM antibodies anti-cytomegalovirus in human serum or plasma by using Enzyme-linked Immunosorbent Assay (ELISA). In results this study revealed that the prevalence of anti-CMV IgG antibodies in primary aborted women 54 (88.5 %) of the cases positive while 3 (4.9 %) positive for anti-CMV IgM and the prevalence of ant-CMV IgG antibodies in pregnant women 83 (91.2%) of the cases positive while 6 (6.6 %) positive for anti-CMV IgM. The seroprevalence of anti-CMV IgG antibodies are higher in pregnant women and primary aborted women between age group (26-30) years with the medium living status which survive in urban residency.

KeyWords: Cytomegalovirus, pregnant women, abortion, anti-CMV IgG and IgM antibodies, ELISA.

How to cite: Huda S. Abdul-Mohammed Al-Rawazq et al. Human Cytomegalovirus Immunoglobulin G (IgG) and Immunoglobulin M (IgM) Seropositivity among Pregnant Women in Baghdad City and Their Relations to the Abortion Factors. Curr Res Microbiol Biotechnol. 5(3) 2017: 1100-1104

Curr Res Microbiol Biotechnol

CRMB :::: Vol.5, No.3, May - June 2017